Human p80-coilin is targeted to sphere organelles in the amphibian germinal vesicle.
نویسندگان
چکیده
منابع مشابه
Structure, expression and chromosomal localization of human p80-coilin gene.
Coiled bodies (CBs) are non-capsular nuclear bodies with a diameter of 0.3-1 micron and appear to be composed of coiled fibrils. Human autoantibodies to CBs recognize an 80-kD nuclear protein highly enriched in CBs, and this protein has been named p80-coilin. CBs are known to assemble and disassemble during the cell cycle, with the highest number of CBs occurring at mid to late G1 where p80-coi...
متن کاملDynamics of coilin in Cajal bodies of the Xenopus germinal vesicle.
Cajal bodies (CBs) are complex organelles found in the nuclei of a wide variety of organisms, including vertebrates, invertebrates, plants, and yeast. In most cell types CBs are <1 microm in diameter, severely limiting the range of experimental observations that can be made on them. By contrast, CBs in the amphibian oocyte nucleus (also called the germinal vesicle) are 2-10 microm in diameter. ...
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Background The utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. The aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. MaterialsAndMethods The study was performed in a private IVF center. 318 germinal vesicle (GV...
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We have examined the distribution of snRNPs in the germinal vesicle (GV) of frogs and salamanders by immunofluorescent staining and in situ nucleic acid hybridization. The major snRNAs involved in pre-mRNA splicing (U1, U2, U4, U5, and U6) occur together in nearly all loops of the lampbrush chromosomes, and in hundreds to thousands of small granules (1-4 microns diameter) suspended in the nucle...
متن کاملHuman autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin
Antibodies producing an unusual immunofluorescent pattern were identified in the sera of patients with diverse autoimmune features. This pattern was characterized by the presence of up to six round discrete nuclear bodies in interphase cell nuclei. Immunoblotting analysis showed that these sera recognized an 80-kD nuclear protein, and affinity-purified anti-p80 antibody from the protein band re...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1994
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.5.10.1119